Overview

  • Product nameAnti-eIF4ENIF1 antibody
    See all eIF4ENIF1 primary antibodies
  • Description
    Rabbit polyclonal to eIF4ENIF1
  • Specificityab55880 specifically recognizes human eIF4ENIF1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    KPQVDRTRDGFRNRQ

    conjugated to KLH, corresponding to amino acids 650-664 of Human eIF4ENIF1

  • Positive control
    • HeLa cytoplasmic or nuclear lysate
  • General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab55880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use 5µl for 1010 cells.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 108 kDa).
    Staining of eIF4ENIF1 in immunoblotting is specifically inhibited by the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
    • Tissue specificityWidely expressed.
    • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2610509L04Rik antibody
      • 4E T antibody
      • 4E-T antibody
      • 4ET antibody
      • 4ET_HUMAN antibody
      • A930019J01Rik antibody
      • AA410001 antibody
      • AU021239 antibody
      • Clast 4 antibody
      • Clast4 antibody
      • D11Ertd166e antibody
      • eIF4E transporter antibody
      • Eif4enif1 antibody
      • Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody
      • Eukaryotic translation initiation factor 4E transporter antibody
      • FLJ21601 antibody
      • FLJ26551 antibody
      • OTTMUSP00000000698 antibody
      • OTTMUSP00000005193 antibody
      • OTTMUSP00000005194 antibody
      • OTTMUSP00000036075 antibody
      see all

    Anti-eIF4ENIF1 antibody images

    • All lanes : Anti-eIF4ENIF1 antibody (ab55880) at 2 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : K562 cell lysate

      Secondary
      HRP conjugated Goat-anti Rabbit IgG
      Developed using the ECL technique

      Predicted band size : 108 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    References for Anti-eIF4ENIF1 antibody (ab55880)

    ab55880 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"