Overview

  • Product name
  • Description
    Rabbit polyclonal to eIF4ENIF1
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    MDRRSMGETESGDAFLDLK-C

    conjugated to KLH, corresponding to N terminal amino acids 1-19 of Human eIF4ENIF1.

  • Positive control
    • HeLa cell lysates HEK-293T cells
  • General notes
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab55881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notes
    IP: Use 5-10 µl, using HeLa cell lysate.
    ICC/IF: Use at a concentration of 5 - 10 µg/ml, using paraformaldehyde-fixed HEK-293T cells.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa using HeLa cell lysate. Staining of eIF4ENIF1 in immunoblotting is specifically inhibited by the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
    • Tissue specificity
      Widely expressed.
    • Cellular localization
      Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2610509L04Rik antibody
      • 4E T antibody
      • 4E-T antibody
      • 4ET antibody
      • 4ET_HUMAN antibody
      • A930019J01Rik antibody
      • AA410001 antibody
      • AU021239 antibody
      • Clast 4 antibody
      • Clast4 antibody
      • D11Ertd166e antibody
      • eIF4E transporter antibody
      • Eif4enif1 antibody
      • Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody
      • Eukaryotic translation initiation factor 4E transporter antibody
      • FLJ21601 antibody
      • FLJ26551 antibody
      • OTTMUSP00000000698 antibody
      • OTTMUSP00000005193 antibody
      • OTTMUSP00000005194 antibody
      • OTTMUSP00000036075 antibody
      see all

    Images

    • Lanes 1, 3 & 4 : Anti-eIF4ENIF1 antibody (ab55881) at 1 µg/ml
      Lane 2 : Anti-eIF4ENIF1 antibody (ab55881) at 2 µg/ml

      Lane 1 : Extracts of HeLa cells
      Lane 2 : Extracts of HeLa cells
      Lane 3 : Extracts of HeLa cells with eIF4ENIF1 immunizing peptide at 10 µg/ml
      Lane 4 : Extracts of HeLa cells with Non-relevant peptide at 10 µg/ml

      Secondary
      HRP-conjugated Goat anti-Rabbit IgG
      Developed using the ECL technique

      Predicted band size : 108 kDa
    • IF localization of eIF4ENIF1 in HEK-293T cells stained with ab55881 at 5 mg/ml (A), and DAPI (B). The antibody was detected with a FITC conjugated Goat anti-Rabbit IgG.
    • All lanes : Anti-eIF4ENIF1 antibody (ab55881) at 1/1000 dilution

      Lane 1 : Whole cell lysate prepared from HeLa cells
      Lane 2 : siRNA knockdown

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 800CW conjugated goat anti-rabbit polyclonal at 1/15000 dilution

      Predicted band size : 108 kDa

      Image courtesy of an anonymous Abreview.

      See Abreview

    References

    This product has been referenced in:
    • Kamenska A  et al. The DDX6-4E-T interaction mediates translational repression and P-body assembly. Nucleic Acids Res 44:6318-34 (2016). Read more (PubMed: 27342281) »
    • Cargnello M  et al. Phosphorylation of the eukaryotic translation initiation factor 4E-transporter (4E-T) by c-Jun N-terminal kinase promotes stress-dependent P-body assembly. Mol Cell Biol 32:4572-84 (2012). Read more (PubMed: 22966201) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (n2a mouse neuroblastoma)
    Loading amount
    30 µg
    Specification
    n2a mouse neuroblastoma
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 18 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    10 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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