Overview

  • Product nameAnti-eIF4ENIF1 antibody
    See all eIF4ENIF1 primary antibodies
  • Description
    Mouse monoclonal to eIF4ENIF1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Baboon
  • Immunogen

    Recombinant fragment: NPRPGTPLHL AMVQQQLQRS VLHPPGSGSH AAAVSVQTTP QNVPSRSGLP HMHSQLEHRP SQRSSSPVGL AKWFGSDVLQ QPLPSMPAKV ISVDELEYRQ, corresponding to amino acids 886 - 985 of human eIF4ENIF1 (NP_062817) with tag.

  • Positive control
    • Partial recombinant human eIF4ENIF1 protein with tag ; eIF4ENIF1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 108 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionNucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
  • Tissue specificityWidely expressed.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610509L04Rik antibody
    • 4E T antibody
    • 4E-T antibody
    • 4ET antibody
    • 4ET_HUMAN antibody
    • A930019J01Rik antibody
    • AA410001 antibody
    • AU021239 antibody
    • Clast 4 antibody
    • Clast4 antibody
    • D11Ertd166e antibody
    • eIF4E transporter antibody
    • Eif4enif1 antibody
    • Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody
    • Eukaryotic translation initiation factor 4E transporter antibody
    • FLJ21601 antibody
    • FLJ26551 antibody
    • OTTMUSP00000000698 antibody
    • OTTMUSP00000005193 antibody
    • OTTMUSP00000005194 antibody
    • OTTMUSP00000036075 antibody
    see all

Anti-eIF4ENIF1 antibody images

  • Anti-eIF4ENIF1 antibody (ab77027) at 1 µg/ml + recombinant human eIF4ENIF1 protein fragment with a 26 kDa tag at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 108 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-eIF4ENIF1 antibody (ab77027) at 1 µg/ml

    Lane 1 : eIF4ENIF1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 108 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)

References for Anti-eIF4ENIF1 antibody (ab77027)

ab77027 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293T, HeLa)
Loading amount 20 µg
Specification 293T, HeLa
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"