Overview

  • Product nameAnti-eIF4G1 antibody
    See all eIF4G1 primary antibodies
  • Description
    Rabbit polyclonal to eIF4G1
  • Tested applicationsSuitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human, African Green Monkey
    Predicted to work with: Mouse, Rabbit, Horse, Hamster, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide (Human) - which represents a portion of human eukaryotic translation InitiationFactor 4 Gamma 1 encoded in part by exons 10 and 11.

  • Positive control
    • Rat liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab2609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/1000.
WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 220 kDa). EIF4G is more susceptible to degradation compared to other proteins, especially from some tissue sources such as liver. This is true even when tissue is stored at frozen. SDS-PAGE sample buffer may improve the stability, but samples that are stored frozen may show degradation bands that have been described in the literature.Therefore if multiple bands are observed in WB, it is likely due to the degradation of eIF4G rather than non-specificity of the antibody.
IHC-P Use a concentration of 4 µg/ml.
ICC/IF 1/500.

Target

  • FunctionComponent of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
  • Involvement in diseaseDefects in EIF4G1 are the cause of Parkinson disease type 18 (PARK18) [MIM:614251]. An autosomal dominant, late-onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
  • Sequence similaritiesBelongs to the eIF4G family.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • Post-translational
    modifications
    Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1.
    Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686A1451 antibody
    • eIF 4 gamma 1 antibody
    • eIF 4G 1 antibody
    • eIF 4G1 antibody
    • eIF-4-gamma 1 antibody
    • eIF-4G 1 antibody
    • eIF-4G1 antibody
    • EIF4 gamma antibody
    • EIF4F antibody
    • EIF4G antibody
    • EIF4G1 antibody
    • EIF4GI antibody
    • Eukaryotic translation initiation factor 4 gamma 1 antibody
    • IF4G1_HUMAN antibody
    • p220 antibody
    see all

Anti-eIF4G1 antibody images



  • Predicted band size : 220 kDa


    50µg (lane 1) and 150µg (lane 2) rat liver lysate, separated on 7.5% acrylamide SDS-PAGE gel. Detected using ab2609 at 1:1000 (A), 1:5000 (B) and 1:10000 (C) dilution by ECL.
  • ab2609 (4µg/ml) staining eIF4G1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm of the intestinal cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab2609 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2609, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-eIF4G1 antibody (ab2609)

This product has been referenced in:
  • Thomas MG  et al. Mammalian Staufen 1 is recruited to stress granules and impairs their assembly. J Cell Sci 122:563-73 (2009). ICC/IF ; African Green Monkey . Read more (PubMed: 19193871) »
  • Morrison JM & Racaniello VR Proteinase 2Apro is essential for enterovirus replication in type I interferon-treated cells. J Virol 83:4412-22 (2009). WB ; Human . Read more (PubMed: 19211759) »

See all 11 Publications for this product

Product Wall

Thank you for your enquiry. The information on the datasheet was from a mail correspondence with the product originator back in June 2004 (please see FAQ section on datasheet). I am afraid that with our current mail system, we can not retrieve any ...

Read More

Thank you for your enquiry. EIF4G is more susceptible to degradation compared to other proteins, especially from some tissue sources such as liver. This is true even when tissue is stored at frozen. SDS-PAGE sample buffer may improve the stability, ...

Read More

I'm very sorry to hear you are having problems with a new vial of ab2609. Thank you for taking the time to fill in the questionnaire, it is very useful for me to understand your problem. I have checked the batches you have used and found out that th...

Read More

As requested we will refund you for the cost of this antibody. I note from our datasheet that this antibody has only been tested for cross reactivity with human and rat and you advise that you were using Baby Hamster Kidney cells. However, the fact tha...

Read More

Thank you for your patience. I'm sorry to hear that you are experiencing trouble with this antibody. I'm trying to obtain more information from the originator to help figure just what may be going on, but am still waiting to hear back. There is no ...

Read More

Thank you for your patience. The originator of ab2609 has provided me with the following information: "eIF-4G migrates in SDS-PAGE just above myosin standard. If colored molecular weight markers are used it can be just above or just below dyed myosin s...

Read More

The peptide used to generate the antibody is common to all 5 isoforms shown for SwissProt entry Q04637 (http://au.expasy.org/cgi-bin/niceprot.pl?Q04637). It lies between residues 560 and 660 of isoform A as defined in SwissProt entry Q04637.

All the information that we have about the immunogen is that it is a synthetic peptide (Human) - which represents a portion of human eukaryotic translation Initiation Factor 4 Gamma 1 encoded in part by exons 10 and 11. I can't predict if it will work ...

Read More

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with rabbit has not yet been tested. Should you decide to go ahead and purchase this product, please l...

Read More

This antibody is recommended for use in WB at a dilution range 1:1000 to 1:10000. The dilution you have tried is right at the edge of the detection limit and I would therefore recommend that you try using it at a lower dilution - 1:1000 or even 1:500. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"