eIF4G1 overexpression 293T lysate (whole cell) (ab94246)

Overview

  • Product nameeIF4G1 overexpression 293T lysate (whole cell)
  • Description
    eIF4G1 overexpression lysate
  • General notesab94246 is a 293T cell transfected lysate in which Human eIF4G1 has been transiently over-expressed using a pCMV-eIF4G1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Disease: Defects in EIF4G1 are the cause of Parkinson disease type 18 (PARK18) [MIM:614251]. An autosomal dominant, late-onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. Similarity: Belongs to the eIF4G family. Contains 1 MI domain. Contains 1 MIF4G domain. Contains 1 W2 domain. PTM: Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1. Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

eIF4G1 overexpression 293T lysate (whole cell) images

  • ab94246 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-eIF4G1 antibody (ab54970) at 1/500 dilution

    Lane 1 : eIF4G1 overexpression 293T lysate (whole cell) (ab94246)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for eIF4G1 overexpression 293T lysate (whole cell) (ab94246)

ab94246 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94246.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"