Anti-eIF4GII antibody - N-terminal (ab155482)

Overview

  • Product name
    Anti-eIF4GII antibody - N-terminal
    See all eIF4GII primary antibodies
  • Description
    Rabbit polyclonal to eIF4GII - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to a region within N-terminal amino acids 1-203 of Human eIF4GII (Uniprot: O43432).

  • Positive control
    • 293T, A431, HeLa, JC, BCL-1 and HepG2 whole cell lysates. Human AGS xenograft tissue.
  • General notes
    Keep as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 177 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.
  • Sequence similarities
    Belongs to the eukaryotic initiation factor 4G family.
    Contains 5 HEAT repeats.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • Post-translational
    modifications
    Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF-4-gamma 3 antibody
    • eIF-4-gamma II antibody
    • eIF-4G 3 antibody
    • eIF4G 3 antibody
    • EIF4G3 antibody
    • eIF4GII antibody
    • Eukaryotic translation initiation factor 4 gamma 3 antibody
    • eukaryotic translation initiation factor 4 gamma, 3 antibody
    • eukaryotic translation initiation factor-4 gamma, 3 antibody
    • IF4G3_HUMAN antibody
    see all

Images

  • All lanes : Anti-eIF4GII antibody - N-terminal (ab155482) at 1/10000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 177 kDa



    5% SDS PAGE
  • All lanes : Anti-eIF4GII antibody - N-terminal (ab155482) at 1/5000 dilution

    Lane 1 : Mouse JC whole cell lysate
    Lane 2 : Mouse BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 177 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human AGS xenograft tissue, labeling eIF4GII using ab155482 at a 1/500 dilution.

References

ab155482 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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