Overview

  • Product name
  • Description
    Rabbit polyclonal to eIF4H
  • Specificity
    Reacts specifically with mouse 27 kDa eIF4 protein
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, a wide range of other species
  • Immunogen

    Synthetic peptide derived from eIF4H protein

Properties

Applications

Our Abpromise guarantee covers the use of ab64323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 27 kDa.
ICC 1/100 - 1/1000.

Target

  • Function
    Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
  • Tissue specificity
    The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
  • Involvement in disease
    Note=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • AU018978 antibody
    • D5Ertd355e antibody
    • E430026L18Rik antibody
    • Ef4h antibody
    • eIF 4H antibody
    • eIF-4H antibody
    • EIF4H antibody
    • Eukaryotic translation initiation factor 4H antibody
    • IF4H_HUMAN antibody
    • KIAA0038 antibody
    • WBSCR1 antibody
    • Williams Beuren syndrome chromosomal region 1 protein homolog antibody
    • Williams Beuren syndrome chromosome region 1 antibody
    • Williams-Beuren syndrome chromosomal region 1 protein antibody
    • Williams-Beuren syndrome chromosome region 1 antibody
    • WSCR1 antibody
    see all

References

This product has been referenced in:
  • Sawhney S  et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . Read more (PubMed: 25643152) »
  • Meijer HA  et al. Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation. Science 340:82-5 (2013). WB . Read more (PubMed: 23559250) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma cell line N2A)
Loading amount
30 µg
Specification
Mouse Neuroblastoma cell line N2A
Gel Running Conditions
Reduced Denaturing (13.5%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HeLA)
Loading amount
40 µg
Specification
HeLA
Gel Running Conditions
Reduced Denaturing (18%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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