Overview

  • Product nameAnti-eIF4H antibody
    See all eIF4H primary antibodies
  • Description
    Rabbit polyclonal to eIF4H
  • Tested applicationsSuitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    A region between residue 198 and 248 of human eIF4H using the numbering given in entry NP_071496.1

  • Positive control
    • HeLa cells lysate 293T cells lysate
  • General notesShelf Life: 1 year from date of receipt

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified using an epitope specific to eIF4H immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab86179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionStimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
    • Tissue specificityThe short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
    • Involvement in diseaseNote=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
    • Cellular localizationCytoplasm > perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • AU018978 antibody
      • D5Ertd355e antibody
      • E430026L18Rik antibody
      • Ef4h antibody
      • eIF 4H antibody
      • eIF-4H antibody
      • EIF4H antibody
      • Eukaryotic translation initiation factor 4H antibody
      • IF4H_HUMAN antibody
      • KIAA0038 antibody
      • WBSCR1 antibody
      • Williams Beuren syndrome chromosomal region 1 protein homolog antibody
      • Williams Beuren syndrome chromosome region 1 antibody
      • Williams-Beuren syndrome chromosomal region 1 protein antibody
      • Williams-Beuren syndrome chromosome region 1 antibody
      • WSCR1 antibody
      see all

    Anti-eIF4H antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling eIF4H with ab86179 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-eIF4H antibody (ab86179) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 27 kDa


      Exposure time : 3 minutes

    References for Anti-eIF4H antibody (ab86179)

    ab86179 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"