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Rabbit polyclonal to eIF4H
Predicted to work with:
Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
A region between residue 198 and 248 of human eIF4H using the numbering given in entry NP_071496.1
HeLa cells lysate
293T cells lysate
Shelf Life: 1 year from date of receipt
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Affinity purified using an epitope specific to eIF4H immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
Is unsuitable for IP.
Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
Involvement in disease
Note=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Contains 1 RRM (RNA recognition motif) domain.
Cytoplasm > perinuclear region.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4H antibody (ab86179)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling eIF4H with ab86179 at 1/200 (1µg/ml). Detection: DAB.
Western blot - eIF4H antibody (ab86179)
All lanes : Anti-eIF4H antibody (ab86179) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 27 kDa Exposure time: 3 minutes
has not yet been referenced specifically in any publications.
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