Overview

  • Product nameAnti-EIF5 antibody
    See all EIF5 primary antibodies
  • Description
    Mouse monoclonal to EIF5
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, Human EIF5 (432 aa) with 26 kDa enzyme tag (AAH32866).

  • Positive control
    • Recombinant full length human EIF5.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged EIF5 is approximately 10ng/ml if used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionCatalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
  • Sequence similaritiesBelongs to the eIF-2-beta/eIF-5 family.
    Contains 1 W2 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810011H21Rik antibody
    • D12Ertd549e antibody
    • EIF 5 antibody
    • EIF 5A antibody
    • eIF-5 antibody
    • Eif5 antibody
    • Eukaryotic initiation factor 5 antibody
    • Eukaryotic translation initiation factor 5 antibody
    • IF5_HUMAN antibody
    • MGC36374 antibody
    • MGC36509 antibody
    see all

Anti-EIF5 antibody images

  • Anti-EIF5 antibody (ab76633) at 5 µg/ml + Recombinant full length human EIF5 with 26 kDa tag, at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 49 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)

References for Anti-EIF5 antibody (ab76633)

ab76633 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"