The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detection limit for recombinant tagged EIF5 is approximately 10ng/ml if used as a capture antibody.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 49 kDa).
FunctionCatalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Sequence similaritiesBelongs to the eIF-2-beta/eIF-5 family. Contains 1 W2 domain.