Overview

  • Product name
  • Description
    Rabbit polyclonal to EIF5
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 100 and 150 of Human EIF5 (NP_892116.2)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab85913 was affinity purified using an epitope specific to EIF5 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-EIF5 antibody (ab85913) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 49 kDa
      Observed band size : 49 kDa

    References

    This product has been referenced in:
    • Tzani I  et al. Systematic analysis of the PTEN 5' leader identifies a major AUU initiated proteoform. Open Biol 6:N/A (2016). Read more (PubMed: 27249819) »
    • Loughran G  et al. Stringency of start codon selection modulates autoregulation of translation initiation factor eIF5. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 22156057) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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