• Product nameAnti-eIF5A antibody
    See all eIF5A primary antibodies
  • Description
    Rabbit polyclonal to eIF5A
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human eIF5A.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HUVEC; Jurkat; MOLT4; MCF7; Caco2; U937. This antibody gave a positive signal in the following tissue: Human lung adenocarcinoma. This antibody gave a positive signal in the following cell types: HeLa, Hek293, HepG2 (Formaldehyde fixed), HepG2, MCF-7 (Methanol fixed).



Our Abpromise guarantee covers the use of ab105903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 17 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionmRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
  • Tissue specificityExpressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
  • Sequence similaritiesBelongs to the eIF-5A family.
  • Post-translational
    eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
  • Cellular localizationCytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF 4D antibody
    • eIF 5A 1 antibody
    • EIF 5A antibody
    • eIF 5A1 antibody
    • eIF-4D antibody
    • eIF-5A antibody
    • eIF-5A-1 antibody
    • eIF-5A1 antibody
    • eIF4D antibody
    • eif5a antibody
    • EIF5A1 antibody
    • eIF5AI antibody
    • Eukaryotic initiation factor 5A antibody
    • Eukaryotic initiation factor 5A isoform 1 antibody
    • Eukaryotic translation initiation factor 5A 1 antibody
    • Eukaryotic translation initiation factor 5A antibody
    • Eukaryotic translation initiation factor 5A-1 antibody
    • IF5A1_HUMAN antibody
    • MGC104255 antibody
    • MGC99547 antibody
    • Rev binding factor antibody
    • Rev-binding factor antibody
    • uORF A antibody
    • uORF antibody
    see all

Anti-eIF5A antibody images

  • All lanes : Anti-eIF5A antibody (ab105903) at 1 µg/ml

    Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • IHC image of eIF5A staining in Human lung cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105903, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab105903 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab105903 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293, and HepG2 cells at 5ug/ml, and in 100% Methanol fixed (5min) HepG2 and MCF-7 cells at 5ug/ml.

References for Anti-eIF5A antibody (ab105903)

ab105903 has not yet been referenced specifically in any publications.

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