Anti-eIF5A antibody (ab137561)
Key features and details
- Rabbit polyclonal to eIF5A
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-eIF5A antibody
See all eIF5A primary antibodies -
Description
Rabbit polyclonal to eIF5A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus tropicalis -
Immunogen
Recombinant full length protein corresponding to Human eIF5A aa 1-154.
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt 4, Raji and Mouse brain cell lysates; HeLa cells; SW480 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa. |
Target
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Function
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. -
Tissue specificity
Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level). -
Sequence similarities
Belongs to the eIF-5A family. -
Post-translational
modificationseIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine). -
Cellular localization
Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. - Information by UniProt
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Database links
- Entrez Gene: 444990 Cow
- Entrez Gene: 1984 Human
- Entrez Gene: 276770 Mouse
- Entrez Gene: 287444 Rat
- Entrez Gene: 448142 Xenopus tropicalis
- Omim: 600187 Human
- SwissProt: Q6EWQ7 Cow
- SwissProt: P63241 Human
see all -
Alternative names
- eIF 4D antibody
- eIF 5A 1 antibody
- EIF 5A antibody
see all
Images
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Anti-eIF5A antibody (ab137561) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE -
Anti-eIF5A antibody (ab137561) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 16 kDa
15% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling eIF5A with ab137561 at 1/200 dilution. Lower image is merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded SW480 xenograft labelling eIF5A with ab137561 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab137561 has been referenced in 3 publications.
- Barba-Aliaga M et al. Yeast Translation Elongation Factor eIF5A Expression Is Regulated by Nutrient Availability through Different Signalling Pathways. Int J Mol Sci 22:N/A (2020). PubMed: 33379337
- Imam S et al. eIF5A inhibition influences T cell dynamics in the pancreatic microenvironment of the humanized mouse model of Type 1 Diabetes. Sci Rep 9:1533 (2019). PubMed: 30733517
- Gao J et al. CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins. J Cell Biol 207:213-23 (2014). PubMed: 25349259