Overview

  • Product name
  • Description
    Rabbit polyclonal to eIF5A
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-104 of Human eIF5A.

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt 4, Raji and Mouse brain cell lysates; HeLa cells; SW480 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab137561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.

Target

  • Function
    mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
  • Tissue specificity
    Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
  • Sequence similarities
    Belongs to the eIF-5A family.
  • Post-translational
    modifications
    eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
  • Cellular localization
    Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF 4D antibody
    • eIF 5A 1 antibody
    • EIF 5A antibody
    • eIF 5A1 antibody
    • eIF-4D antibody
    • eIF-5A antibody
    • eIF-5A-1 antibody
    • eIF-5A1 antibody
    • eIF4D antibody
    • eif5a antibody
    • EIF5A1 antibody
    • eIF5AI antibody
    • Eukaryotic initiation factor 5A antibody
    • Eukaryotic initiation factor 5A isoform 1 antibody
    • Eukaryotic translation initiation factor 5A 1 antibody
    • Eukaryotic translation initiation factor 5A antibody
    • Eukaryotic translation initiation factor 5A-1 antibody
    • IF5A1_HUMAN antibody
    • MGC104255 antibody
    • MGC99547 antibody
    • Rev binding factor antibody
    • Rev-binding factor antibody
    • uORF A antibody
    • uORF antibody
    see all

Images

  • Anti-eIF5A antibody (ab137561) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 16 kDa
  • Anti-eIF5A antibody (ab137561) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 16 kDa
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling eIF5A with ab137561 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft labelling eIF5A with ab137561 at 1/500 dilution.

References

This product has been referenced in:
  • Gao J  et al. CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins. J Cell Biol 207:213-23 (2014). Read more (PubMed: 25349259) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137561.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up