Overview

  • Product name
  • Description
    Mouse polyclonal to eIF5A2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Chimpanzee, Rhesus monkey, Chinese hamster
  • Immunogen

    Full length protein, corresponding to amino acids 1-153 of Human eIF5A2 (NP_065123.1)

  • Positive control
    • eIF5A2 transfected 293T cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab168122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
  • Tissue specificity
    Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
  • Sequence similarities
    Belongs to the eIF-5A family.
  • Post-translational
    modifications
    eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
  • Cellular localization
    Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9630038B20 antibody
    • eIF 5A 2 antibody
    • eIF 5A2 antibody
    • eIF-4D antibody
    • eIF-5A-2 antibody
    • eIF-5A2 antibody
    • EIF5A 2 antibody
    • EIF5A2 antibody
    • eIF5AII antibody
    • Eukaryotic initiation factor 5A isoform 2 antibody
    • Eukaryotic translation initiation factor 5A-2 antibody
    • Eukaryotic translation initiation factor 5A2 antibody
    • IF5A2_HUMAN antibody
    see all

Images

  • All lanes : Anti-eIF5A2 antibody (ab168122) at 1 µg/ml

    Lane 1 : eIF5A2 Transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG

    Predicted band size: 17 kDa

  • Immunofluorescence of HeLa cells labeling eIF5A2 using ab168122 at 10 µg/ml.

References

ab168122 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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