Overview

  • Product name
    Anti-eIF5A2 antibody [EPR7411]
    See all eIF5A2 primary antibodies
  • Description
    Rabbit monoclonal [EPR7411] to eIF5A2
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acid residues in Human eIF5A2.

  • Positive control
    • 293T, HeLa, A431, Caco 2 and HepG2 cell lysates; Human ovarian carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-eIF5A2 antibody [EPR7411] (ab150403)

    Anti-eIF5A2 antibody (Alexa Fluor® 488) [EPR7411] (ab208130)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.

Target

  • Function
    mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
  • Tissue specificity
    Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
  • Sequence similarities
    Belongs to the eIF-5A family.
  • Post-translational
    modifications
    eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
  • Cellular localization
    Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9630038B20 antibody
    • eIF 5A 2 antibody
    • eIF 5A2 antibody
    • eIF-4D antibody
    • eIF-5A-2 antibody
    • eIF-5A2 antibody
    • EIF5A 2 antibody
    • EIF5A2 antibody
    • eIF5AII antibody
    • Eukaryotic initiation factor 5A isoform 2 antibody
    • Eukaryotic translation initiation factor 5A-2 antibody
    • Eukaryotic translation initiation factor 5A2 antibody
    • IF5A2_HUMAN antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab126735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126735, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-eIF5A2 antibody [EPR7411] (ab126735) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : CaCo 2 cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat-anti-rabbit HRP at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 17 kDa
  • ab126735 at 1/100 dilution, staining eIF5A2 in Formalin fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Meng QB  et al. Overexpression of eukaryotic translation initiation factor 5A2 (EIF5A2) correlates with cell aggressiveness and poor survival in gastric cancer. PLoS One 10:e0119229 (2015). Read more (PubMed: 25793713) »
  • Qin X  et al. Inhibition of eIF5A results in aberrant uterine natural killer cell function and embryo loss in mice. Am J Reprod Immunol 71:229-40 (2014). Read more (PubMed: 24382123) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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