Recombinant
RabMAb

Anti-eIF5A2 antibody [EPR7412] (ab126733)

Overview

  • Product name
    Anti-eIF5A2 antibody [EPR7412]
    See all eIF5A2 primary antibodies
  • Description
    Rabbit monoclonal [EPR7412] to eIF5A2
  • Specificity
    The antibody may react with not only A2 but also A1.
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acid residues of Human eIF5A2.

  • Positive control
    • HepG2 and Caco 2 cell lysates; Human ovarian carcinoma tissue; HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
    • Tissue specificity
      Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
    • Sequence similarities
      Belongs to the eIF-5A family.
    • Post-translational
      modifications
      eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
    • Cellular localization
      Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
    • Information by UniProt
    • Database links
    • Alternative names
      • 9630038B20 antibody
      • eIF 5A 2 antibody
      • eIF 5A2 antibody
      • eIF-4D antibody
      • eIF-5A-2 antibody
      • eIF-5A2 antibody
      • EIF5A 2 antibody
      • EIF5A2 antibody
      • eIF5AII antibody
      • Eukaryotic initiation factor 5A isoform 2 antibody
      • Eukaryotic translation initiation factor 5A-2 antibody
      • Eukaryotic translation initiation factor 5A2 antibody
      • IF5A2_HUMAN antibody
      see all

    Images

    • ab126733 at 1/100 dilution, staining eIF5A2 in Formalin-fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.
    • ab126733, at 1/100 dilution, staining eLF5A2 in HepG2 cells by Immunofluorescence (green). DAPI counterstain (blue).
    • All lanes : Anti-eIF5A2 antibody [EPR7412] (ab126733) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 17 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126733 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting us. The antibody ab150439 is tested and is very specific for eIF5A-2. Regarding eIF5A-1 (otherwise known as eIF5A) many of these antibodies have some amount of cross-reactivity, although one predicted to be least likely to cros...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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