Recombinant
RabMAb

Anti-eIF5A2 antibody [EPR7412] (ab150439)

Overview

  • Product name
    Anti-eIF5A2 antibody [EPR7412]
    See all eIF5A2 primary antibodies
  • Description
    Rabbit monoclonal [EPR7412] to eIF5A2
  • Host species
    Rabbit
  • Specificity
    Minimal cross-reactivity is observed with eIF5A1.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF5A2 aa 100-200. The exact sequence is proprietary.

  • Positive control
    • HepG2, HeLa, 293T and Raji cell lysates; Human colon adenocarcinoma and testis tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
    • Tissue specificity
      Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
    • Sequence similarities
      Belongs to the eIF-5A family.
    • Post-translational
      modifications
      eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
    • Cellular localization
      Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
    • Information by UniProt
    • Database links
    • Alternative names
      • 9630038B20 antibody
      • eIF 5A 2 antibody
      • eIF 5A2 antibody
      • eIF-4D antibody
      • eIF-5A-2 antibody
      • eIF-5A2 antibody
      • EIF5A 2 antibody
      • EIF5A2 antibody
      • eIF5AII antibody
      • Eukaryotic initiation factor 5A isoform 2 antibody
      • Eukaryotic translation initiation factor 5A-2 antibody
      • Eukaryotic translation initiation factor 5A2 antibody
      • IF5A2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-eIF5A2 antibody [EPR7412] (ab150439) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP labelled antibody at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling eIF5A2 with ab150439 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling eIF5A2 with ab150439 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150439 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150439 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150439 showing +ve staining.

    References

    This product has been referenced in:
    • Yao M  et al. N1-guanyl-1, 7-diaminoheptane enhances the sensitivity of pancreatic ductal adenocarcinoma cells to gemcitabine via the inhibition of eukaryotic translation initiation factor 5A2. Exp Ther Med 14:2101-2107 (2017). Read more (PubMed: 28962130) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for contacting us. The antibody ab1504389 is tested and is very specific for eIF5A-2. Regarding eIF5A-1 (othereise known as eIF5A) many of these antibodies have some amount of cross-reactivity, although one predicted to be least likely to cro...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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