Overview

  • Product nameAnti-EIF5B antibody
    See all EIF5B primary antibodies
  • Description
    Mouse polyclonal to EIF5B
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human EIF5B (AAH32639.1)

  • Positive control
    • Humna placenta tissue lysate, jurkat cell lysate, EIF5B transfected 293T cell lysate and HeLa cell

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab89016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 139 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFunction in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.
    • Sequence similaritiesBelongs to the IF-2 family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp434I036 antibody
      • eIF 5B antibody
      • eIF-5B antibody
      • Eif5b antibody
      • Eukaryotic translation initiation factor 5B antibody
      • FLJ10524 antibody
      • IF2 antibody
      • IF2P_HUMAN antibody
      • KIAA0741 antibody
      • Translation initiation factor IF 2 antibody
      • Translation initiation factor IF-2 antibody
      • Translation initiation factor IF2 antibody
      see all

    Anti-EIF5B antibody images

    • Anti-EIF5B antibody (ab89016) at 5 µg/ml + Human placenta tissue lysate at 50 µg

      Predicted band size : 139 kDa
    • Anti-EIF5B antibody (ab89016) at 5 µg/ml + Jurkat cell lysate at 50 µg

      Predicted band size : 139 kDa
    • All lanes : Anti-EIF5B antibody (ab89016) at 5 µg/ml

      Lane 1 : EIF5B transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 139 kDa
    • ab89016 at 10µg/ml staining EIF5B on HeLa cell by immunofluorescence

    References for Anti-EIF5B antibody (ab89016)

    ab89016 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for your inquiry. Unfortunately we have not any information on the ab89016 or the ab74958 in regards to testing on other species. I have however provided here below the links to the alignement, showing relative good hope that they c...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"