Anti-EIF5B antibody (ab89016)
Key features and details
- Mouse polyclonal to EIF5B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-EIF5B antibody
See all EIF5B primary antibodies -
Description
Mouse polyclonal to EIF5B -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human EIF5B. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
Positive control
- Humna placenta tissue lysate, jurkat cell lysate, EIF5B transfected 293T cell lysate and HeLa cell
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 139 kDa.
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 139 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
-
Function
Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. -
Sequence similarities
Belongs to the IF-2 family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 9669 Human
- Omim: 606086 Human
- SwissProt: O60841 Human
- Unigene: 158688 Human
-
Alternative names
- DKFZp434I036 antibody
- eIF 5B antibody
- eIF-5B antibody
see all
Images
-
Anti-EIF5B antibody (ab89016) at 5 µg/ml + Human placenta tissue lysate at 50 µg
Predicted band size: 139 kDa -
Anti-EIF5B antibody (ab89016) at 5 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 139 kDa -
All lanes : Anti-EIF5B antibody (ab89016) at 5 µg/ml
Lane 1 : EIF5B transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 139 kDa -
ab89016 at 10µg/ml staining EIF5B on HeLa cell by immunofluorescence
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab89016 has not yet been referenced specifically in any publications.