Anti-EKLF / KLF1 antibody - ChIP Grade (ab2483)

Overview

  • Product nameAnti-EKLF / KLF1 antibody - ChIP Grade
    See all EKLF / KLF1 primary antibodies
  • Description
    Goat polyclonal to EKLF / KLF1 - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    ATAETALPSISTLT

    , corresponding to N terminal amino acids 2-15 of Human KLF1.

Properties

Applications

Our Abpromise guarantee covers the use of ab2483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution. PubMed: 19074263
WB Use at an assay dependent dilution. Preliminary experiments gave an approx 52kDa band in K562 cell lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 38kDa according to NP_006554. The 52kDa band was successfully blocked by incubation with the immunizing peptide

Target

  • FunctionTranscription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
  • Tissue specificityExpression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
  • Involvement in diseaseDefects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4) [MIM:613673]. It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.
    Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.
    Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.
  • Cellular localizationNucleus. Colocalizes with SUMO1 in nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDAN4 antibody
    • EKLF antibody
    • EKLF PEN antibody
    • Erythroid krueppel like transcription factor (EKLF) (Erythroid transcription factor) antibody
    • Erythroid krueppel-like transcription factor antibody
    • Erythroid Kruppel like factor antibody
    • Erythroid specific transcription factor EKLF antibody
    • HBFQTL6 antibody
    • Human erythroid-specific transcription factor EKLF mRNA complete cds antibody
    • INLU antibody
    • Klf1 antibody
    • KLF1_HUMAN antibody
    • Krueppel-like factor 1 antibody
    • Kruppel like factor 1 (erythroid) antibody
    see all

Anti-EKLF / KLF1 antibody - ChIP Grade images

  • Detection of EKLF / KLF1 binding at the region upstream of the transcription start site of the HBE1 gene by ChIP with ab2483. Chromatin from CD43+ cells was cross-linked prior to ChIP using ab2483 at a concentration of 0.2 µg/µg chromatin. DNA was quantified using RT-PCR. Please see abreview by Genpathway Inc for additional details.

References for Anti-EKLF / KLF1 antibody - ChIP Grade (ab2483)

This product has been referenced in:
  • Forand A  et al. EKLF-driven PIT1 expression is critical for mouse erythroid maturation in vivo and in vitro. Blood 121:666-78 (2013). Read more (PubMed: 23190530) »
  • Pang CJ  et al. Kruppel-like factor 1 (KLF1), KLF2, and Myc control a regulatory network essential for embryonic erythropoiesis. Mol Cell Biol 32:2628-44 (2012). Read more (PubMed: 22566683) »

See all 6 Publications for this product

Product Wall

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I have looked in this and it appears that the wrong image was placed on the Webpage for ab2483, however we do guarantee the antibody to work in ChIP, as the correct image can be found at the link below:
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Detection step Real-time PCR
Sample Human Cell lysate - nuclear (CD34+ cells)
Specification CD34+ cells
Negative control Region on chromosome 5 (Untr5) that is far from any known gene annotation and not expected to be bound by KLF1
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control Region upstream of the transcription start site of the HBE1 gene
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Mr. Genpathway Inc

Verified customer

Submitted Jul 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"