Overview

  • Product name
  • Description
    Rabbit polyclonal to ELAC1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide from within residues: LFHRIPSFGF SVVEKKRPGK LNAQKLKDLG VPPGPAYGKL KNGISVVLEN , corresponding to internal sequence amino acids 179-228 of Human ELAC1 (NP_061166)

  • Positive control
    • Fetal Human heart lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

  • Function
    Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.
  • Tissue specificity
    Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the RNase Z family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • D29 antibody
    • Deleted in Ma29 antibody
    • ELAC 1 antibody
    • ElaC homolog 1 (E. coli) antibody
    • ElaC homolog 1 antibody
    • ElaC homolog protein 1 antibody
    • ELAC1 antibody
    • Ribonuclease Z 1 antibody
    • RNase Z 1 antibody
    • RNZ1_HUMAN antibody
    • tRNA 3 endonuclease 1 antibody
    • tRNA 3' processing endoribonuclease antibody
    • tRNA Z (short form) antibody
    • tRNA Z antibody
    • tRNase Z 1 antibody
    • tRNase ZS antibody
    • Zinc phosphodiesterase ELAC protein 1 antibody
    see all

Anti-ELAC1 antibody images

  • Anti-ELAC1 antibody (ab83252) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References for Anti-ELAC1 antibody (ab83252)

ab83252 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83252.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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