Purification notesIgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/200 - 1/1000. 1.0 µg of elastase (porcine pancreas) in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionEfficient protease with alanine specificity but only little elastolytic activity.
Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.