Elastins pooled from pig, human, dog chicken, rat and cow.
Our Abpromise guarantee covers the use of ab21610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20138661|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22884813|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22140132|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||1/1000 - 1/2000.|
Immunohistochemical analysis of Bouin’s solution fixed (25cm H2O pressure for 90 min) paraffin-embedded wild type mouse lung (12mo, female) tissue labeling Elastin with ab21610 at 1/50 dilution, followed by HRP-conjugated secondary antibody.
Left Panel: No antibody control.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH-3T3 cells labeling Elastin with ab21610 at 1/200 dilution, followed by a FITC-conjugated secondary antibody. The nuclear counter stain is DAPI (blue).