• Product name
  • Description
    Rabbit polyclonal to Elastin
  • Host species
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, ICC/IF, IHC-P, ELISAmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Dog, Human, Pig
  • Immunogen

    Elastins pooled from pig, human, dog chicken, rat and cow.



Our Abpromise guarantee covers the use of ab21610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20138661
IHC-Fr Use at an assay dependent concentration. PubMed: 22884813
ICC/IF Use at an assay dependent concentration. PubMed: 22140132
IHC-P Use at an assay dependent concentration.
ELISA 1/1000 - 1/2000.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
    • Tissue specificity
      Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
    • Involvement in disease
      Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
      Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
      Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Sequence similarities
      Belongs to the elastin family.
    • Post-translational
      Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
      Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
    • Information by UniProt
    • Database links
    • Alternative names
      • Elastin antibody
      • ELN antibody
      • ELN_HUMAN antibody
      • SVAS antibody
      • Tropoelastin antibody
      • WBS antibody
      • WS antibody
      see all


    • Immunohistochemical analysis of Bouin’s solution fixed (25cm H2O pressure for 90 min) paraffin-embedded wild type mouse lung (12mo, female) tissue labeling Elastin with ab21610 at 1/50 dilution, followed by HRP-conjugated secondary antibody.

      Left Panel: No antibody control.

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH-3T3 cells labeling Elastin with ab21610 at 1/200 dilution, followed by a FITC-conjugated secondary antibody. The nuclear counter stain is DAPI (blue).

    • Immunohistochemical analysis of frozen microfibrous vascular grafts from rat carotid artery tissue, staining Elastin with ab21610.


    This product has been referenced in:
    • Fang CL  et al. Liposome-Encapsulated Baicalein Suppressed Lipogenesis and Extracellular Matrix Formation in Hs68 Human Dermal Fibroblasts. Front Pharmacol 9:155 (2018). Read more (PubMed: 29559910) »
    • Shirali AS  et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). Read more (PubMed: 29615716) »

    See all 23 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Mouse Tissue sections (Prostate)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Nov 28 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674)
    Yes - 0.05% tween 20
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

    Mr. David Ivancic

    Verified customer

    Submitted May 22 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Fibrotic Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
    Fibrotic Skin
    Blocking step
    Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    10% NBF

    Premier Laboratory

    Verified customer

    Submitted Apr 05 2016

    Thank you for contacting us and your interest in our products.
    The three antibodies you mentioned would all be expected to recognise the extracellular elastin. The mouse monoclonals ab9519 and ab97963 are of the same clone (BA-4) and has been shown...

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    Thank you for your enquiry.

    I can confirm that ab21640 and ab9519 are sold as whole antiseruma and ascites respectively. Unpurified antibodies, such as these sold as whole antiserum, ascites or tissue culture supernatant will not have a conc...

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    Thank you for contacting us.
    Your credit note ID is ****.
    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purcha...

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    Thank you for your message. I am sorry to hear you have previouslyhad difficultywith two of our antibodies.

    I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible for us to keep small sample sizes of...

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    Thank you for contacting Abcam.
    I am sorry about all of the issues you are having with ab21610 in western blotting. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse samples. Since I do no...

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    Thank you for contacting Abcam regarding ab21610.

    I can confirm that this antibody will also react with tropoelastin.

    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questi...

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    Thank you for your inquiry.

    The lab said that they would think ab21607 & ab21610 would recognize the precursor of elastin, aka tropoelastin, but this hasn't been specifically tested.

    I think I found an antibody that would work for...

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