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Full length native elastin purified from human skin.
Our Abpromise guarantee covers the use of ab23747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections labeling Elastin with ab23747 at 1/400 dilution. Tissue was fixed with formaldehyde and permeabilized with EDTA pH9, for 20 min at 100°C. An undiluted HRP conjugated goat anti-rabbit secondary antibody was used.