• Product nameAnti-Elastin antibody
    See all Elastin primary antibodies
  • Description
    Rabbit polyclonal to Elastin
  • Specificityab23747 reacts with elastin, cross-reactivity in RIA at 1:500 dilution: human collagen type I, II, III, IV, V <0.1 %; human fibronectin < 0.1%
  • Tested applicationsSuitable for: IHC-P, ELISA, RIA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native elastin purified from human skin.

  • Positive control
    • In Western Blot, this antibody gave a positive signal in human skin and human lung tissue lysates.



Our Abpromise guarantee covers the use of ab23747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
ICC/IF Use at an assay dependent concentration.


  • FunctionMajor structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • Tissue specificityExpressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • Involvement in diseaseDefects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similaritiesBelongs to the elastin family.
  • Post-translational
    Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Elastin antibody
    • ELN antibody
    • ELN_HUMAN antibody
    • SVAS antibody
    • Tropoelastin antibody
    • WBS antibody
    • WS antibody
    see all

Anti-Elastin antibody images

  • IHC image of ab23747 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23747 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Elastin antibody (ab23747) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human lung normal tissue lysate - total protein (40 - 65 years) (ab30281)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-Elastin antibody (ab23747)

This product has been referenced in:
  • Tian X  et al. Adipose stem cells promote smooth muscle cells to secrete elastin in rat abdominal aortic aneurysm. PLoS One 9:e108105 (2014). WB ; Rat . Read more (PubMed: 25243605) »

See 1 Publication for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH9, 20 min at 100C
Permeabilization No

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Submitted Jun 13 2011