Overview

  • Product nameAnti-Elastin antibody [BA-4]
    See all Elastin primary antibodies
  • Description
    Mouse monoclonal [BA-4] to Elastin
  • Tested applicationsSuitable for: WB, IP, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Sheep, Goat, Guinea pig, Cat, Dog, Human, Pig
  • Immunogen

    Bovine alpha-Elastin.

  • Positive control
    • This antibody gave a positive signal in human skin tissue lysate.
  • General notes


    Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab9519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IP 1/100 - 1/500.
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100. 1/50 - 1/100 (ABC method). Incubate primary antibody for 1 hr minimum, at RT.

Target

  • FunctionMajor structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • Tissue specificityExpressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • Involvement in diseaseDefects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similaritiesBelongs to the elastin family.
  • Post-translational
    modifications
    Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Elastin antibody
    • ELN antibody
    • ELN_HUMAN antibody
    • SVAS antibody
    • Tropoelastin antibody
    • WBS antibody
    • WS antibody
    see all

Anti-Elastin antibody [BA-4] images

  • ab9519 staining pig small intestine tissue sections by IHC-P.  Sections were fixed with Bouins and subjected to an enzymatic antigen retreival step.  The primary antibody was diluted 1/200 in a commercially available antibody diluent (replacing the blocking step) and incubated with the sample for 1 hour at 20°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Anti-Elastin antibody [BA-4] (ab9519) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab9519 staining Elastin in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.

References for Anti-Elastin antibody [BA-4] (ab9519)

This product has been referenced in:
  • Hinata N  et al. Coexistence of elastic fibers with hyaluronic acid in the human urethral sphincter complex: a histological study. J Urol 190:1313-9 (2013). Human . Read more (PubMed: 23583858) »
  • Weinbaum JS  et al. The matrix-binding domain of microfibril-associated glycoprotein-1 targets active connective tissue growth factor to a fibroblast-produced extracellular matrix. Macromol Biosci 10:1338-44 (2010). Read more (PubMed: 20799254) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Specification skin
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 10 2016

Thank you for your enquiry.

I can confirm that ab21640 and ab9519 are sold as whole antiseruma and ascites respectively. Unpurified antibodies, such as these sold as whole antiserum, ascites or tissue culture supernatant will not have a conc...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Lung Artery)
Specification Lung Artery
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Prot K
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Jul 11 2012

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab9519 in rat. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit...

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I am very pleased to hear you would like to accept our offer and test ab9519 in mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the Ad...

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Vielen Dank für Ihren Anruf.


Ich habe einen Proteinsequenz Vergleich zwischen den Immunogenen derMaus Klone [BA-4] und[10B8] (ab9519, ab97963und ab77804)durchgeführt, und folgendes hat sich ergeben: Die Übereinstimmung zw...

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Merci de votre appel et de votre intérêt pour nos anticorps contre l'élastine. Comme discuté au téléphone, nous n'avons malheureusement pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester un de nos anticorps...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital i...

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Thanks for your reply. I'm sorry but the specific enzyme used for antigen retrieval with ab9519 is not known. I think it would be useful to try pepsin or trypsin as well. Also, what type of sample tissue are you working with?

Thank you for your enquiry. I just want to make sure I understand your question. You are wondering what enzyme should be conjugated to your secondary antibody for use in IHC-P? If that is the case, most commonly researchers use HRP. For u...

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