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Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues.
Our Abpromise guarantee covers the use of ab9519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).|
|IP||1/100 - 1/500.|
|IHC-P||1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||1/50 - 1/100. 1/50 - 1/100 (ABC method). Incubate primary antibody for 1 hr minimum, at RT.|
ab9519 staining pig small intestine tissue sections by IHC-P. Sections were fixed with Bouins and subjected to an enzymatic antigen retreival step. The primary antibody was diluted 1/200 in a commercially available antibody diluent (replacing the blocking step) and incubated with the sample for 1 hour at 20°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.