Overview

  • Product name
  • Description
    Rabbit polyclonal to ELAVL2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ELAVL2 aa 155-184 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q12926

  • Positive control
    • K562 cell line lysate; Human brain tissue; K562 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.
  • Tissue specificity
    Brain; neural-specific.
  • Sequence similarities
    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELAV like neuron specific RNA binding protein 2 antibody
    • ELAV like Neuronal Protein 1 antibody
    • ELAV like Protein 2 antibody
    • ELAV-like neuronal protein 1 antibody
    • ELAV-like protein 2 antibody
    • ELAV2_HUMAN antibody
    • ELAVL2 antibody
    • HEL-N1 antibody
    • HELN1 antibody
    • HU Antigen B antibody
    • Hu-antigen B antibody
    • HuB antibody
    • Nervous System Specific RNA Binding Protein MelN1 antibody
    • Nervous system-specific RNA-binding protein Hel-N1 antibody
    see all

Images

  • Anti-ELAVL2 antibody (ab171414) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size: 40 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling ELAVL2 with ab171414 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometric analysis of K562 cells labeling ELAVL2 with ab171414 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab171414 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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