Anti-ELAVL2 + ELAVL4 antibody (ab72603)

Overview

  • Product nameAnti-ELAVL2 + ELAVL4 antibody
    See all ELAVL2 + ELAVL4 primary antibodies
  • Description
    Rabbit polyclonal to ELAVL2 + ELAVL4
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human ELAVL2 + ELAVL4.

  • Positive control
    • Extracts from HUVEC cells and Jurkat cells. HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notesELISA: 1/10000.
    ICC/IF: 1/500 - 1/1000.
    WB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weights: 40 and 42 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceELAV2 binds RNA and seems to recognize a GAAA motif and is neuronal specific. ELAVL4 may play a role in neuron-specific RNA processing. It contains 3 RNA recognition motif (RRM) domains and is localised to brain tissue. ELAVL4 has been linked with the disease, paraneoplastic encephalomyelitis sensory neuropathy, which is associated with small cell lung cancer and is characterized by dementia, sensory loss, and other neurological disabilities. This disease may result from an immune response primarily directed against a small cell lung tumor antigen which is misdirected against similar antigens expressed in brain (such as ELAVL4).
    • Database links
    • Alternative names
      • ELAV like 4 antibody
      • ELAV like neuronal protein 1 antibody
      • ELAV like protein 2 antibody
      • ELAV like protein 4 antibody
      • ELAVL 2 antibody
      • ELAVL 4 antibody
      • HELN1 antibody
      • Hu antigen B antibody
      • Hu antigen D antibody
      • HUB antibody
      • HUD antibody
      see all

    Anti-ELAVL2 + ELAVL4 antibody images

    • All lanes : Anti-ELAVL2 + ELAVL4 antibody (ab72603) at 1/500 dilution

      Lane 1 : Extracts from HUVEC cells
      Lane 2 : Extracts from Jurkat cells
      Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 40 , 42 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 75 kDa,80 kDa,85 kDa. We are unsure as to the identity of these extra bands.
    • ab72603, at 1/500 dilution, staining HepG2 cells in the absence or presence of the immunising peptide.

    References for Anti-ELAVL2 + ELAVL4 antibody (ab72603)

    ab72603 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cultured Cells (NPCs derived from iPSC)
    Permeabilization Yes - 0.25% Triton X-100 in PBS
    Specification NPCs derived from iPSC
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 10 2016

    Thank you for your reply and sorry for the delay in mine.



    I have aligned our exact immunogen sequence for ab136069 with the provided protein sequences. The homology score is listed below:

    Dogfish Elavl4: 92.9% identity in a ...

    Read More

    I can now share some further information with you in regards to the other anti-ELAVL2 + ELAVL4 antibody we have on our catalogue, ab136069.

    The immunogen used to raise this antibody has been taken from within the residues 50-100 of human ELAV2...

    Read More

    Many thanks for contacting us and your interest in our products.



    The exact immunogen used to raise the Anti-ELAVL2 + ELAVL4 antibody (ab72603) is proprietary information. However, I am able to share with you that the immunogen used ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"