Overview

  • Product name
  • Description
    Goat polyclonal to ELF5
  • Specificity
    This antibody is expected to recognize both reported isoforms (as represented by NP_001413; NP_938195)
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: GKNAHGWQEDKL, corresponding to C terminal amino acids 244-255 of Human ELF5.

  • General notes
    GenBank Accession Number - NP_001413; NP_938195

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation.
    Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGA[AT]. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters.
  • Tissue specificity
    Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, kidney and prostate. Weakly expressed in placenta and lung. Isoform 1 and isoform 2 are differentially expressed in different tissues. In the kidney, only isoform 1 was expressed, while prostate expressed both isoforms, with levels of isoform 2 being higher. Expression is up-regulated during keratinocyte differentiation. Several epithelial carcinoma cell lines showed lack of expression.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Domain
    The PNT domain acts as a transcriptional activator.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E74 like factor 5 (ets domain transcription factor) antibody
    • E74 like factor 5 antibody
    • E74-like factor 5 antibody
    • ELF 5 antibody
    • ELF5 antibody
    • ELF5_HUMAN antibody
    • Epithelium restricted ESE 1 related Ets factor antibody
    • Epithelium restricted ESE1 related Ets factor antibody
    • Epithelium specific Ets transcription factor 2 antibody
    • Epithelium-restricted ESE-1-related Ets factor antibody
    • Epithelium-specific Ets transcription factor 2 antibody
    • ESE 2 antibody
    • ESE-2 antibody
    • ESE2 antibody
    • Ets domain TF antibody
    • Ets domain transcription factor antibody
    • ETS related transcription factor Elf 5 antibody
    • ETS related transcription factor Elf5 antibody
    • ETS-related transcription factor Elf-5 antibody
    see all

Images

  • ab13581 at 4 µg/ml staining ELF5 in Human thyroid gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

References

This product has been referenced in:
  • Chakrabarti R  et al. Elf5 inhibits the epithelial-mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2. Nat Cell Biol 14:1212-22 (2012). IHC ; Human . Read more (PubMed: 23086238) »
  • Krueger F  et al. Down-regulation of Cdx2 in colorectal carcinoma cells by the Raf-MEK-ERK 1/2 pathway. Cell Signal 21:1846-56 (2009). Read more (PubMed: 19686845) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab13581.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up