Overview

  • Product nameAnti-ELF5 antibody
    See all ELF5 primary antibodies
  • Description
    Mouse monoclonal to ELF5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment: SRTSLQSSHL WEFVRDLLLS PEENCGILEW EDREQGIFRV VKSEALAKMW GQRKKNDRMT YEKLSRALRY YYKTGILERV DRRLVYKFGK NAHGWQED, corresponding to amino acids 166-264 of Human ELF5 (NP_938195) with tag.

  • Positive control
    • ELF5 transfected 293T cell lysate. Recombinant tagged ELF5 fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionTranscriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation.
    Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGA[AT]. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters.
  • Tissue specificityExpressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, kidney and prostate. Weakly expressed in placenta and lung. Isoform 1 and isoform 2 are differentially expressed in different tissues. In the kidney, only isoform 1 was expressed, while prostate expressed both isoforms, with levels of isoform 2 being higher. Expression is up-regulated during keratinocyte differentiation. Several epithelial carcinoma cell lines showed lack of expression.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • DomainThe PNT domain acts as a transcriptional activator.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E74 like factor 5 (ets domain transcription factor) antibody
    • E74 like factor 5 antibody
    • E74-like factor 5 antibody
    • ELF 5 antibody
    • ELF5 antibody
    • ELF5_HUMAN antibody
    • Epithelium restricted ESE 1 related Ets factor antibody
    • Epithelium restricted ESE1 related Ets factor antibody
    • Epithelium specific Ets transcription factor 2 antibody
    • Epithelium-restricted ESE-1-related Ets factor antibody
    • Epithelium-specific Ets transcription factor 2 antibody
    • ESE 2 antibody
    • ESE-2 antibody
    • ESE2 antibody
    • Ets domain TF antibody
    • Ets domain transcription factor antibody
    • ETS related transcription factor Elf 5 antibody
    • ETS related transcription factor Elf5 antibody
    • ETS-related transcription factor Elf-5 antibody
    see all

Anti-ELF5 antibody images

  • Anti-ELF5 antibody (ab77007) at 1 µg/ml + Recombinant tagged ELF5 fragment at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 31 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ELF5 antibody (ab77007) at 1 µg/ml

    Lane 1 : ELF5 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-ELF5 antibody (ab77007)

ab77007 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"