Overview

  • Product name
  • Description
    Rabbit polyclonal to ELK1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    WSTLSPIAPRSPAK-C

    , with a C-terminal Cysteine, corresponding to amino acids 379-382 of Human ELK1.

  • General notes
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000. This concentration is recommended using Elk1 fusion protein. Predicted molecular weight: 45 kDa. This antibody detects total Elk1, independent of phosphorylation state.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
    • Tissue specificity
      Lung and testis.
    • Sequence similarities
      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • Post-translational
      modifications
      Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
      On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ELK 1 antibody
      • Elk1 antibody
      • ELK1 member of ETS oncogene family antibody
      • ELK1 protein antibody
      • ELK1, ETS transcription factor antibody
      • ELK1_HUMAN antibody
      • ELK2 member of ETS oncogene family antibody
      • ETS domain containing protein Elk 1 antibody
      • ETS domain containing protein Elk1 antibody
      • ETS domain protein Elk1 antibody
      • ETS domain-containing protein Elk-1 antibody
      • ETS like gene 1 antibody
      • Member of ETS oncogene family antibody
      • Oncogene Elk1 antibody
      • Tyrosine kinase (ELK1) oncogene antibody
      see all

    References

    This product has been referenced in:
    • Al-Sadi R  et al. TNF-a Modulation of Intestinal Epithelial Tight Junction Barrier Is Regulated by ERK1/2 Activation of Elk-1. Am J Pathol 183:1871-84 (2013). Read more (PubMed: 24121020) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for your enquiry. The concentration of ab11847 is 75 ug/ml, and the concentration of ab11848 is 20 ug/ml. If you have any more questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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