Overview

  • Product nameAnti-ELK1 antibody
    See all ELK1 primary antibodies
  • Description
    Rabbit polyclonal to ELK1
  • Specificityab31431 detects endogenous levels of Elk-1 protein around Serine 389
  • Tested applicationsSuitable for: IHC-P, IP, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human Elk-1 around the phosphorylation site of Serine 389.

  • Positive control
    • Breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab31431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • FunctionStimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • Tissue specificityLung and testis.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Anti-ELK1 antibody images

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma, using anti-Elk1 antibody (ab31431, diluted 1:100)

References for Anti-ELK1 antibody (ab31431)

ab31431 has not yet been referenced specifically in any publications.

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For testing ab31431 in ChIP
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Thank you for your telephone call yesterday afternoon.

As discussed on the telephone, as far as we are aware,ab31431 has never been tested in ChIP. All tested applications and speciesare specified on our datasheets, and these are updated as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"