• Product nameAnti-ELK1 antibody [E277] - ChIP Grade
    See all ELK1 primary antibodies
  • Description
    Rabbit monoclonal [E277] to ELK1 - ChIP Grade
  • SpecificityThis antibody does not cross react with other ETS family members
  • Tested applicationsSuitable for: WB, IHC-P, ICC, IP, Flow Cyt, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in C terminus of human ELK1.

  • Epitopeab32106 reacts with an epitope located in the C terminal region of ELK1.
  • Positive control
    • Human breast carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab32106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50.
ICC 1/100.
IP 1/80.
Flow Cyt 1/20.
ChIP Use at an assay dependent concentration. PubMed: 22589737


  • FunctionStimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • Tissue specificityLung and testis.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1, ETS transcription factor antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Anti-ELK1 antibody [E277] - ChIP Grade images

  • Anti-ELK1 antibody [E277] - ChIP Grade (ab32106) at 1/500 dilution

    Predicted band size : 45 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded human breast carcinoma using ab32106 at a dilution of 1/50
  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ELK1 with unpurified ab32106 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

References for Anti-ELK1 antibody [E277] - ChIP Grade (ab32106)

This product has been referenced in:
  • Aguilar-Martinez E  et al. The ubiquitin ligase UBE3A dampens ERK pathway signalling in HPV E6 transformed HeLa cells. PLoS One 10:e0119366 (2015). Read more (PubMed: 25815718) »
  • Sillivan SE  et al. ELK1 transcription factor linked to dysregulated striatal mu opioid receptor signaling network and OPRM1 polymorphism in human heroin abusers. Biol Psychiatry 74:511-9 (2013). IP ; Human . Read more (PubMed: 23702428) »

See all 12 Publications for this product

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The concentration of this lot is 0.2180 mg/ml