Anti-ELK1 (phospho S389) antibody (ab28818)


  • Product nameAnti-ELK1 (phospho S389) antibody
    See all ELK1 primary antibodies
  • Description
    Rabbit polyclonal to ELK1 (phospho S389)
  • Specificityab28818 recognises phospho-ELK-1(Ser389).
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phospho-peptide derived from human ELK-1 around the phosphorylation site of Serine 389.

  • Positive control
    • Breast carcinoma.



Our Abpromise guarantee covers the use of ab28818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 45 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • FunctionStimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • Tissue specificityLung and testis.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1, ETS transcription factor antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Anti-ELK1 (phospho S389) antibody images

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-ELK-1(Ser389) ab28818; Right: The same antibody preincubated with synthesized phosphopeptide.
  • ab28818 staining ELK1 (phospho S389) in human bladder transitional cell carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/50) for 1 hour at 20°C. An undiluted HRP-conjugated Mouse anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-ELK1 (phospho S389) antibody (ab28818)

ab28818 has not yet been referenced specifically in any publications.

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Thank you for your enquiry and your interest in our products.

I regret to inform that you that the blocking peptide (neither the immunizing peptides) are commercially available.


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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human bladder transitional cell carcinoma)
Specification human bladder transitional cell carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No

Dr. Imran Ahmad

Verified customer

Submitted Nov 22 2010