Anti-ELK1 (phospho T417) antibody (ab28817)

Overview

  • Product name
    Anti-ELK1 (phospho T417) antibody
    See all ELK1 primary antibodies
  • Description
    Rabbit polyclonal to ELK1 (phospho T417)
  • Specificity
    ab28817 recognises Phospho-ELK-1(Thr417).
  • Tested applications
    Suitable for: ELISA, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phospho-peptide derived from human ELK-1 around the phosphorylation site of Threonine 417.

  • Positive control
    • Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab28817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • Tissue specificity
    Lung and testis.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1, ETS transcription factor antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-ELK-1(Thr417) Antibody (ab28817); Right: The same antibody preincubated with synthesized phosphopeptide.

References

ab28817 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (A431 Cells)
Specification
A431 Cells
Treatment
30uM etoposide for 30 hrs
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 17 2013

Thank you for your enquiry and your interest in our products.

I regret to inform that you that the blocking peptide (neither the immunizing peptides) are commercially available.

Apologies!

If you need any further assistanc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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