The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detection limit for recombinant tagged ELK3 is approximately 0.3ng/ml when used as a capture antibody.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
FunctionMay be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of the Fos serum response element.
Sequence similaritiesBelongs to the ETS family. Contains 1 ETS DNA-binding domain.