Overview

  • Product nameAnti-ELK3 antibody
    See all ELK3 primary antibodies
  • Description
    Rabbit polyclonal to ELK3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 251-300 (LPSEHRSLFL EAACHDSDSL EPLNLSSGSK TKSPSLPPKA KKPKGLEISA) of Human ELK3 (NP_005221)

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-ELK3 antibody images

  • Anti-ELK3 antibody (ab83186) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ELK3 antibody (ab83186)

ab83186 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount 15 µg
Specification NIH 3T3 cells
Gel Running Conditions Non-reduced Denaturing (4-12% gradient Bis-Tris gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Jan 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"