Overview

  • Product name
    Anti-ELK4 antibody
    See all ELK4 primary antibodies
  • Description
    Rabbit polyclonal to ELK4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region withinynthetic peptide: VAPLSPARLQ GANTLFQFPS VLNSHGPFTL SGLDGPSTPG PFSPDLQKT, corresponding to C terminal amino acids 383-431 of Human ELK4

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Function
    Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • Elk4 antibody
      • ELK4_HUMAN antibody
      • ETS domain containing protein Elk4 antibody
      • ETS domain protein antibody
      • ETS domain-containing protein Elk-4 antibody
      • SAP-1 antibody
      • SAP1 antibody
      • Serum response factor accessory protein 1 antibody
      • SRF accessory protein 1 antibody
      • TCF1 antibody
      see all

    Images

    • Western blot - Anti-ELK4 antibody (ab49244)
      Western blot - Anti-ELK4 antibody (ab49244)
      Anti-ELK4 antibody (ab49244) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk in PBS.

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 47 kDa
      Observed band size: 41 kDa (why is the actual band size different from the predicted?)



      Gel concentration: 12%

    References

    ab49244 has not yet been referenced specifically in any publications.

    Publishing research using ab49244? Please let us know so that we can cite the reference in this datasheet.

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