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Overview

  • Product name
    Anti-ELK4 antibody
    See all ELK4 primary antibodies
  • Description
    Rabbit polyclonal to ELK4
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region withinynthetic peptide: VAPLSPARLQ GANTLFQFPS VLNSHGPFTL SGLDGPSTPG PFSPDLQKT, corresponding to C terminal amino acids 383-431 of Human ELK4

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Function
    Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • Elk4 antibody
      • ELK4_HUMAN antibody
      • ETS domain containing protein Elk4 antibody
      • ETS domain protein antibody
      • ETS domain-containing protein Elk-4 antibody
      • SAP-1 antibody
      • SAP1 antibody
      • Serum response factor accessory protein 1 antibody
      • SRF accessory protein 1 antibody
      • TCF1 antibody
      see all

    Anti-ELK4 antibody images

    • Western blot - ELK4 antibody (ab49244)
      Western blot - ELK4 antibody (ab49244)
      Anti-ELK4 antibody (ab49244) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk in PBS.

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 47 kDa
      Observed band size : 41 kDa (why is the actual band size different from the predicted?)

    References for Anti-ELK4 antibody (ab49244)

    ab49244 has not yet been referenced specifically in any publications.

    Publishing research using ab49244? Please let us know so that we can cite the reference in this datasheet.

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