Anti-ELK4 antibody (ab86002)
Key features and details
- Rabbit polyclonal to ELK4
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-ELK4 antibody -
Description
Rabbit polyclonal to ELK4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Three synthetic peptides derived from human ELK4, conjugated to KLH.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate) -
Concentration information loading...
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Purity
Whole antiserum -
Purification notes
Crude serum, final bleed -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 47 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 47 kDa. |
Target
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Function
Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- Elk4 antibody
- ELK4_HUMAN antibody
- ETS domain containing protein Elk4 antibody
see all
Images
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All lanes : Anti-ELK4 antibody (ab86002) at 1/1000 dilution (in 5% non-fat milk-PBST solution incubated for 60 minutes)
Lane 1 : uninduced culture of E. coli (plus anti thio at 1/5000 dilution)
Lane 2 : induced culture of E. coli (plus anti thio at 1/5000 dilution)
Secondary
All lanes : anti-Rabbit IgG HRP conjugated incubated for 60 minutes at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
Gel concentration: 10%
The actual band molecular weight is the result of the target protein ELK4 (47 kDa) attached to a tag (14 kDa).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab86002 has been referenced in 1 publication.
- Mao X et al. ELK4-mediated lncRNA SNHG22 promotes gastric cancer progression through interacting with EZH2 and regulating miR-200c-3p/Notch1 axis. Cell Death Dis 12:957 (2021). PubMed: 34663788