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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum
Purity Whole antiserum
Purification notes Crude serum, final bleed
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Direct ELISA: 1/25000.
WB: 1/1000. Predicted molecular weight: 47 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
Sequence similarities Belongs to the ETS family. Contains 1 ETS DNA-binding domain.
Cellular localization Nucleus.
Information by UniProt
ETS domain containing protein Elk4 antibody
Anti-ELK4 antibody images
Western blot - ELK4 antibody (ab86002)
All lanes : Anti-ELK4 antibody (ab86002) at 1/1000 dilution (in 5% non-fat milk-PBST solution incubated for 60 minutes) Lane 1 : uninduced culture of E. coli (plus anti thio at 1/5000 dilution)
Lane 2 : induced culture of E. coli (plus anti thio at 1/5000 dilution) Secondary anti-Rabbit IgG HRP conjugated incubated for 60 minutes at 1/1000 dilution Predicted band size : 47 kDa Observed band size : 61 kDa ( why is the actual band size different from the predicted?)
References for Anti-ELK4 antibody (ab86002)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"