Overview

  • Product name
  • Description
    Rabbit polyclonal to ELL2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 400-450 of Human ELL2 (NP_036213.2).

  • Positive control
    • Hela whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab115027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.
  • Sequence similarities
    Belongs to the ELL/occludin family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELL related RNA polymerase II elongation factor antibody
    • Ell2 antibody
    • ELL2_HUMAN antibody
    • Elongation factor for RNA polymerase II 2 antibody
    • Elongation factor RNA for polymerase II 2 antibody
    • Elongation factor RNA polymerase II 2 antibody
    • RNA polymerase II elongation factor ELL2 antibody
    see all

Images

  • All lanes : Anti-ELL2 antibody (ab115027) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 72 kDa


    Exposure time : 30 seconds
  • Detection of ELL2 by Western Blot of Immunprecipitate.
    ab115027, at 1 µg/ml, staining ELL2 in Hela whole cell lysate immunoprecipitated using ab115027 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab115027 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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