Overview

  • Product nameAnti-ELMO1 antibody
    See all ELMO1 primary antibodies
  • Description
    Rabbit polyclonal to ELMO1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human ELMO1.

    (Peptide available as ab72858.)

  • Positive control
    • This antibody gave a positive signal in Jurkat and MOLT4 Whole Cell Lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab72859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

Target

  • FunctionInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
  • Tissue specificityWidely expressed, with a higher expression in the spleen and placenta.
  • Sequence similaritiesContains 1 ELMO domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated by HCK.
  • Cellular localizationCytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
  • Information by UniProt
  • Database links
  • Alternative names
    • CED 12 antibody
    • Ced 12 homolog 1 antibody
    • Ced 12 homolog antibody
    • CED-12 antibody
    • CED12 antibody
    • Ced12 homolog 1 antibody
    • Ced12 homolog antibody
    • ELMO 1 antibody
    • ELMO-1 antibody
    • Elmo1 antibody
    • ELMO1_HUMAN antibody
    • Engulfment and cell motility 1 antibody
    • Engulfment and cell motility protein 1 antibody
    • KIAA0281 antibody
    • MGC126406 antibody
    • Protein ced-12 homolog antibody
    see all

Anti-ELMO1 antibody images

  • All lanes : Anti-ELMO1 antibody (ab72859) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 50 kDa,73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-ELMO1 antibody (ab72859)

ab72859 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Jurkat Ms Brain Ms Spleen Ms Testis)
Loading amount 20 µg
Specification Jurkat Ms Brain Ms Spleen Ms Testis
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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Verified customer

Submitted May 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"