Anti-ELMO1 antibody [ELMO1-6] - C-terminal (ab214069)

Overview

  • Product name
    Anti-ELMO1 antibody [ELMO1-6] - C-terminal
    See all ELMO1 primary antibodies
  • Description
    Mouse monoclonal [ELMO1-6] to ELMO1 - C-terminal
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human ELMO1 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q92556

  • Positive control
    • Jurkat , Raji, Ramos, EL4 and A20 cell line lysates; Raji whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab214069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 84 kDa.
IP Use at an assay dependent concentration.

Use 5-10 µg.

Target

  • Function
    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
  • Tissue specificity
    Widely expressed, with a higher expression in the spleen and placenta.
  • Sequence similarities
    Contains 1 ELMO domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated by HCK.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
  • Information by UniProt
  • Database links
  • Alternative names
    • CED 12 antibody
    • Ced 12 homolog 1 antibody
    • Ced 12 homolog antibody
    • CED-12 antibody
    • CED12 antibody
    • Ced12 homolog 1 antibody
    • Ced12 homolog antibody
    • ELMO 1 antibody
    • ELMO-1 antibody
    • Elmo1 antibody
    • ELMO1_HUMAN antibody
    • Engulfment and cell motility 1 antibody
    • Engulfment and cell motility protein 1 antibody
    • KIAA0281 antibody
    • MGC126406 antibody
    • Protein ced-12 homolog antibody
    see all

Images

  • All lanes : Anti-ELMO1 antibody [ELMO1-6] - C-terminal (ab214069) at 5 µg/ml

    Lane 1 : Jurkat cell line lysate
    Lane 2 : Raji cell line lysate
    Lane 3 : Ramos cell line lysate
    Lane 4 : EL4 cell line lysate
    Lane 5 : A20 cell line lysate

    Secondary
    Goat Anti-Mouse IgG-Peroxidase
    Developed using the ECL technique

    Predicted band size : 84 kDa
  • ab214069 was used to immunoprecipitate ELMO1 from RAJI whole cell extract. Detection antibody: Rabbit Anti-ELMO1 (C-terminal)

    Lane 1: 5µg ab214069 

    Lane 2: 10µg ab214069 

    Lane 3: Negative control - no primary antibody

    Lane 4: Positive control - Raji whole cell extract

References

ab214069 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab214069.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up