Recombinant
RabMAb

Anti-ELMO1 antibody [EPR12919] - BSA and Azide free (ab222227)

Overview

  • Product name
    Anti-ELMO1 antibody [EPR12919] - BSA and Azide free
    See all ELMO1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12919] to ELMO1 - BSA and Azide free
  • Tested applications
    Suitable for: Flow Cyt, IP, ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ELMO1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q92556

  • Positive control
    • Human placenta and Jurkat lysates.
  • General notes

    ab222227 is a PBS-only buffer formulated version of ab174298, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab174298 for information on protocols, dilutions, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
    • Tissue specificity
      Widely expressed, with a higher expression in the spleen and placenta.
    • Sequence similarities
      Contains 1 ELMO domain.
      Contains 1 PH domain.
    • Post-translational
      modifications
      Phosphorylated by HCK.
    • Cellular localization
      Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
    • Information by UniProt
    • Database links
    • Alternative names
      • CED 12 antibody
      • Ced 12 homolog 1 antibody
      • Ced 12 homolog antibody
      • CED-12 antibody
      • CED12 antibody
      • Ced12 homolog 1 antibody
      • Ced12 homolog antibody
      • ELMO 1 antibody
      • ELMO-1 antibody
      • Elmo1 antibody
      • ELMO1_HUMAN antibody
      • Engulfment and cell motility 1 antibody
      • Engulfment and cell motility protein 1 antibody
      • KIAA0281 antibody
      • MGC126406 antibody
      • Protein ced-12 homolog antibody
      see all

    Images

    • This Flow Cyt data was generated using the same atni-ELMO1 antibody clone, EPR12919, in a different buffer formulation (cat# ab174298).

      Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1:200 dilution(1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • This IP data was generated using the same atni-ELMO1 antibody clone, EPR12919, in a different buffer formulation (cat# ab174298).

      Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab174298 at 1/10 dilution.

    References

    ab222227 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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