The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Widely expressed, with a higher expression in the spleen and placenta.
Contains 1 ELMO domain. Contains 1 PH domain.
Phosphorylated by HCK.
Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
Western blot - Anti-ELMO1 antibody [EPR12919] (HRP) (ab204386)
All lanes : Anti-ELMO1 antibody [EPR12919] (HRP) (ab204386) at 1/5000 dilution
Lane 1 : Human placenta tissue lysate - total protein (ab29745) Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 84 kDa Observed band size : 84 kDa
Exposure time : 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204386 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.