Recombinant
RabMAb

Anti-ELMO1 antibody [EPR12919] (HRP) (ab204386)

Overview

  • Product name
    Anti-ELMO1 antibody [EPR12919] (HRP)
    See all ELMO1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12919] to ELMO1 (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ELMO1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q92556

  • Positive control
    • WB: Human placenta tissue and Jurkat whole cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab204386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

Target

  • Function
    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
  • Tissue specificity
    Widely expressed, with a higher expression in the spleen and placenta.
  • Sequence similarities
    Contains 1 ELMO domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated by HCK.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
  • Information by UniProt
  • Database links
  • Alternative names
    • CED 12 antibody
    • Ced 12 homolog 1 antibody
    • Ced 12 homolog antibody
    • CED-12 antibody
    • CED12 antibody
    • Ced12 homolog 1 antibody
    • Ced12 homolog antibody
    • ELMO 1 antibody
    • ELMO-1 antibody
    • Elmo1 antibody
    • ELMO1_HUMAN antibody
    • Engulfment and cell motility 1 antibody
    • Engulfment and cell motility protein 1 antibody
    • KIAA0281 antibody
    • MGC126406 antibody
    • Protein ced-12 homolog antibody
    see all

Images

  • All lanes : Anti-ELMO1 antibody [EPR12919] (HRP) (ab204386) at 1/5000 dilution

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 84 kDa


    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204386 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab204386 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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