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Goat polyclonal to ELMO2
This antibody is expected to recognise both human isoforms.
Predicted to work with:
Mouse, Rat, Cow, Dog, Pig
Synthetic peptide: PKEPSSYDFVYHYG, corresponding to C terminal amino acids 707-720 of Human ELMO2.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
Concentration information loading...
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration. PubMed: 24446484
Use a concentration of 1 - 3 µg/ml. Can be blocked with
Human ELMO2 peptide (ab22879).
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Widely expressed, with a higher expression in skeletal muscle, kidney and placenta.
Contains 1 ELMO domain.
Contains 1 PH domain.
Cytoplasm. Cytoplasm > cytosol. Membrane.
Information by UniProt
CED 12 antibody
Ced 12 homolog 2 antibody
CED 12 homolog A antibody
Western blot - Anti-ELMO2 antibody (ab2240)
Ab2240 staining (2µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELMO2 antibody (ab2240)
ab2240 at 3.8 µg/ml staining ELMO2 in Human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"