ELMO2 overexpression 293T lysate (whole cell) (ab94207)

Overview

  • Product name
    ELMO2 overexpression 293T lysate (whole cell)
  • Description
    ELMO2 overexpression lysate
  • General notes
    ab94207 is a 293T cell transfected lysate in which Human ELMO2 has been transiently over-expressed using a pCMV-ELMO2 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Tissue specificity: Widely expressed, with a higher expression in skeletal muscle, kidney and placenta. Similarity: Contains 1 ELMO domain. Contains 1 PH domain.

Applications

Our Abpromise guarantee covers the use of ab94207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94207 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ELMO2 antibody (ab57557) at 1/500 dilution

    Lane 1 : ELMO2 overexpression 293T lysate (whole cell) (ab94207)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94207 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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