The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2-5µg/mg of lysate.
WB: 1/2,000 - 1/10,000. Detects bands of approximately 71, 81 and 100 kDa (predicted molecular weight: 87 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
RNA polymerase II transcription factor SIII subunit A1 antibody
SIII p110 antibody
TCEB 3 antibody
Transcription elongation factor B (SIII) polypeptide 3 (110kD) antibody
Transcription elongation factor B (SIII) polypeptide 3 antibody
Transcription elongation factor B alpha subunit antibody
Transcription elongation factor B polypeptide 3 antibody
Anti-Elongin A antibody images
Western blot - Elongin A antibody (ab72245)
All lanes : Anti-Elongin A antibody (ab72245) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation - Elongin A antibody (ab72245)
Immunoprecipitation/ Western Blot of Elongin A.
Lane 1: ab72245 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72245 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 1 second.
References for Anti-Elongin A antibody (ab72245)
has not yet been referenced specifically in any publications.
Publishing research using ab72245? Please let us know so that we can cite the reference in this datasheet.
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