Overview

  • Product nameAnti-Elongin A antibody
    See all Elongin A primary antibodies
  • Description
    Rabbit polyclonal to Elongin A
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 325 and 375 of human Elongin A, (NP_003189.1)

  • Positive control
    • Whole cell lysate from Hela and 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab72245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2,000 - 1/10,000. Detects bands of approximately 71, 81 and 100 kDa (predicted molecular weight: 87 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
    • Sequence similaritiesContains 1 F-box domain.
      Contains 1 TFIIS N-terminal domain.
    • DomainThe elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ela1 antibody
      • Elo A antibody
      • EloA antibody
      • ELOA1_HUMAN antibody
      • Elongin 110 kDa subunit antibody
      • Elongin-A antibody
      • ElonginA antibody
      • RNA polymerase II transcription factor SIII subunit A1 antibody
      • SIII antibody
      • SIII p110 antibody
      • TCEB 3 antibody
      • Tceb3 antibody
      • TCEB3A antibody
      • Transcription elongation factor B (SIII) polypeptide 3 (110kD) antibody
      • Transcription elongation factor B (SIII) polypeptide 3 antibody
      • Transcription elongation factor B alpha subunit antibody
      • Transcription elongation factor B polypeptide 3 antibody
      see all

    Anti-Elongin A antibody images

    • All lanes : Anti-Elongin A antibody (ab72245) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 87 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 71 kDa,81 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of Elongin A.
      Lane 1: ab72245 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.

      ab72245 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Detection:Chemiluminescence with an exposure time of 1 second.

    References for Anti-Elongin A antibody (ab72245)

    ab72245 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab72245.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"