Overview

  • Product name
  • Description
    Rabbit polyclonal to Elongin A
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Medaka fish
  • Immunogen

    Synthetic peptide corresponding to a region between residue 725 and the C-terminus (residue 772) of human Elongin A, (NP_003189).

  • Positive control
    • Whole cell lysate from Hela and 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
  • Sequence similarities
    Contains 1 F-box domain.
    Contains 1 TFIIS N-terminal domain.
  • Domain
    The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ela1 antibody
    • Elo A antibody
    • EloA antibody
    • ELOA1_HUMAN antibody
    • Elongin 110 kDa subunit antibody
    • Elongin-A antibody
    • ElonginA antibody
    • RNA polymerase II transcription factor SIII subunit A1 antibody
    • SIII antibody
    • SIII p110 antibody
    • TCEB 3 antibody
    • Tceb3 antibody
    • TCEB3A antibody
    • Transcription elongation factor B (SIII) polypeptide 3 (110kD) antibody
    • Transcription elongation factor B (SIII) polypeptide 3 antibody
    • Transcription elongation factor B alpha subunit antibody
    • Transcription elongation factor B polypeptide 3 antibody
    see all

Images

  • All lanes : Anti-Elongin A antibody (ab72246) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 87 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of Elongin A.
    Lane 1: ab72246 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.

    ab72246 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:Chemiluminescence with an exposure time of 30 seconds.

References

ab72246 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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