The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa).
Use at 2-5 µg/mg of lysate.
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
RNA polymerase II transcription factor SIII subunit A1 antibody
SIII p110 antibody
TCEB 3 antibody
Transcription elongation factor B (SIII) polypeptide 3 (110kD) antibody
Transcription elongation factor B (SIII) polypeptide 3 antibody
Transcription elongation factor B alpha subunit antibody
Transcription elongation factor B polypeptide 3 antibody
Western blot - Elongin A antibody (ab72246)
All lanes : Anti-Elongin A antibody (ab72246) at 0.4 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation - Elongin A antibody (ab72246)
Immunoprecipitation/ Western Blot of Elongin A.
Lane 1: ab72246 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72246 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds.