Overview

  • Product nameAnti-Elongin A antibody
    See all Elongin A primary antibodies
  • Description
    Mouse monoclonal to Elongin A
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 107-216 of human Elongin A protein (NP_003189) with a proprietary tag.

  • Positive control
    • HeLa nuclear extract, transfected 293T cell lysate, Hela cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab88087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 87 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionSIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
  • Sequence similaritiesContains 1 F-box domain.
    Contains 1 TFIIS N-terminal domain.
  • DomainThe elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ela1 antibody
    • Elo A antibody
    • EloA antibody
    • ELOA1_HUMAN antibody
    • Elongin 110 kDa subunit antibody
    • Elongin-A antibody
    • ElonginA antibody
    • RNA polymerase II transcription factor SIII subunit A1 antibody
    • SIII antibody
    • SIII p110 antibody
    • TCEB 3 antibody
    • Tceb3 antibody
    • TCEB3A antibody
    • Transcription elongation factor B (SIII) polypeptide 3 (110kD) antibody
    • Transcription elongation factor B (SIII) polypeptide 3 antibody
    • Transcription elongation factor B alpha subunit antibody
    • Transcription elongation factor B polypeptide 3 antibody
    see all

Anti-Elongin A antibody images

  • Anti-Elongin A antibody (ab88087) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 87 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-Elongin A antibody (ab88087) at 5 µg/ml + HeLa nuclear extract at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 87 kDa
    Observed band size : 87 kDa
  • All lanes : Anti-Elongin A antibody (ab88087) at 5 µg/ml

    Lane 1 : transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 87 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent analysis of ab88087 on HeLa cell. Antibody concentration 10 µg/ml.

References for Anti-Elongin A antibody (ab88087)

ab88087 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"