Anti-ELOVL2 antibody - N-terminal (ab95295)

Overview

  • Product nameAnti-ELOVL2 antibody - N-terminal
    See all ELOVL2 primary antibodies
  • Description
    Rabbit polyclonal to ELOVL2 - N-terminal
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH from the N-terminal region of Human ELOVL2 (NP_060240.3)

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab95295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 35 kDa.

Target

  • FunctionCondensing enzyme that catalyzes the synthesis of polyunsaturated very long chain fatty acid (C20- and C22-PUFA). Acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C20:4(n-6) acyl-CoA.
  • Tissue specificityLiver and testis.
  • Sequence similaritiesBelongs to the ELO family.
  • DomainThe di-lysine motif may confer endoplasmic reticulum localization.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 keto acyl CoA synthase ELOVL2 antibody
    • 3-keto acyl-CoA synthase Elovl2 antibody
    • Elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast) like 2 antibody
    • Elongation of very long chain fatty acids protein 2 antibody
    • ELOV2_HUMAN antibody
    • ELOVL fatty acid elongase 2 antibody
    • Elovl2 antibody
    • FLJ20334 antibody
    • OTTHUMP00000016029 antibody
    • SSC2 antibody
    see all

Anti-ELOVL2 antibody - N-terminal images

  • Anti-ELOVL2 antibody - N-terminal (ab95295) at 1/100 dilution (TTBS (Tween-Tris Buffered Saline: 0.1% Tween-20 in 100 mM Tris-CL [pH 7.5], 0.9% NaCl)/1% NFDM (nonfat dried milk)) + HepG2 cell lysate at 35 µg with NFDM freshly made in TTBS at 10 %

    Secondary
    Enzyme-linked secondary antibody (typically horseradish peroxidase or alkaline phosphatase) in TTBS/1% NFDM for 30 min. at room temperature

    Predicted band size : 35 kDa

References for Anti-ELOVL2 antibody - N-terminal (ab95295)

ab95295 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Testis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Trs/HCl 0.01M pH9, pressure cooker
Permeabilization Yes - PBS-0.1% Triton
Specification Testis
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative Formalin
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Submitted May 11 2015

Application Western blot
Sample Cow Tissue lysate - whole (Liver, muscle, adipose tissue)
Loading amount 50 µg
Specification Liver, muscle, adipose tissue
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
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Submitted Jun 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"