Overview

  • Product nameAnti-ELYS antibody
    See all ELYS primary antibodies
  • Description
    Rabbit polyclonal to ELYS
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa2220 and aa2302 of human ELYS using the numbering given in entry AAN65622.1 (GeneID 25909).

  • Positive control
    • HeLa cells nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab14431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use 2 to 5 ug/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 272 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
    Prepare working dilution immediately before use.
  • Target

    • FunctionRequired for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.
    • Sequence similaritiesBelongs to the ELYS family.
      Contains 1 A.T hook DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm. Nucleus matrix. Chromosome > centromere > kinetochore. Nucleus > nucleoplasm. Nucleus > nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase.
    • Information by UniProt
    • Database links
    • FormThere are 2 isoforms produced by alternative splicing.
    • Alternative names
      • ahctf1 antibody
      • AT hook containing transcription factor 1 antibody
      • DKFZp434N093 antibody
      • ELYS antibody
      • ELYS transcription factor like protein TMBS62 antibody
      • ELYS_HUMAN antibody
      • Embryonic large molecule derived from yolk sac antibody
      • MST108 antibody
      • MSTP108 antibody
      • Protein ELYS antibody
      • Protein MEL-28 antibody
      • Putative AT-hook-containing transcription factor 1 antibody
      • TMBS62 antibody
      see all

    Anti-ELYS antibody images

    • All lanes : Anti-ELYS antibody (ab14431) at 0.5 µg/ml

      Lane 1 : Cell lysates prepared from Hela cells at 10 µg/ml
      Lane 2 : Cell lysates prepared from Hela cells at 20 µg/ml


      Predicted band size : 272 kDa

    References for Anti-ELYS antibody (ab14431)

    ab14431 has not yet been referenced specifically in any publications.

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