Use at an assay dependent concentration. PubMed: 24312579
FunctionNon-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
Involvement in diseaseDefects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
Post-translational modificationsCleaved by caspase-7 in response to apoptosis to produce EMAP-II.
Cellular localizationNucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
Small inducible cytokine subfamily E member 1 antibody
Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody
Small inducible cytokine subfamily E, member 1, formerly antibody
Anti-EMAP II antibody images
Western blot - Anti-EMAP II antibody (ab96506)
All lanes : Anti-EMAP II antibody (ab96506) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 34 kDa 12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-EMAP II antibody (ab96506)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96506 at 1:200 dilution. Image 2: Merged with DNA probe.
References for Anti-EMAP II antibody (ab96506)
This product has been referenced in:
Park SJ et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One10:e0142253 (2015).
Read more (PubMed: 26544075) »
Kim K et al. Reinvestigation of aminoacyl-TRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. PLoS One8:e81734 (2013).
Read more (PubMed: 24312579) »